Engineering of Escherichia coli for Krebs cycle-dependent production of malic acid
نویسندگان
چکیده
منابع مشابه
Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant.
NAD(+)-dependent malic enzyme was cloned from the Escherichia coli genome by PCR based on the published partial sequence of the gene. The enzyme was overexpressed and purified to near homogeneity in two chromatographic steps and was analyzed kinetically in the forward and reverse directions. The Km values determined in the presence of saturating cofactor and manganese ion were 0.26 mM for malat...
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BACKGROUND Understanding the metabolism of the microbial host is essential for the development and optimization of whole-cell based biocatalytic processes, as it dictates production efficiency. This is especially true for redox biocatalysis where metabolically active cells are employed because of the cofactor/cosubstrate regenerative capacity endogenous in the host. Recombinant Escherichia coli...
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15 صفحه اولMalic Enzyme of Escherichia coli
The TPN+-specitk malic enzyme from Escherichia coli has been purified from malate-grown cells. An approximately loo-fold purified preparation is activated by NH4+ and K+ ions. The enzyme is inhibited by acetyl coenzyme A, oxalacetate, TPNH, and DPNH in an allosteric manner. Glycine at concentration ranges above 0.5 M has been shown to activate the enzyme as well as to desensitize it reversibly ...
متن کاملfacilitating lexical access for the fluent production of speech
the hypothesis is that recent and frequent exposure to lexical items leads to a more fluent production of speech in terms of rate of speech. to test the hypothesis , a one- way anova experimental design was carried out. 24 senior student of efl participated in a one-way interview test. data analyses revealed that those who were exposed frequently to the lexical items over a week prior to inte...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2018
ISSN: 1475-2859
DOI: 10.1186/s12934-018-0959-y